Fig 3. Killing of diverse S. aureus clinical isolates by human immune sera in CP5/CP8 OPA assays.

Opsonophagocytic activity of clinical sera from ten vaccinated subjects (Pre: prevaccination bleed and Post: 29 days post vaccination bleed) were determined against CP5 and CP8 isolates representing the four most prevalent clonal complexes. Bars reflect geometric mean OPA titers from sera of ten immunized subjects tested in two separate experiments with 95% confidence intervals (error bars). CP specificity of the OPAs was demonstrated with anti-CP antibody depleted sera (CP5/8 +) generated as described in the Material and Methods section.