Table 1.

Data collection and refinement statistics.

	T4L-EspG1 M. kansasii PDB 5VBA	EspG3 M. marinum PDB 5DLB	EspG3 M. smegmatis PDB 4L4W	EspG3 M. smegmatis PDB 4RCL	EspG3 M. smegmatis PDB 5SXL
Data collection
Wavelength (Å)	1.0000	1.0702	0.9793	1.0000	1.0000
Space group	P212121	C2	C2221	P43212	P3221
Cell dimensions:
a, b, c (Å)	64.14, 81.69,	112.21, 46.02,	47.08, 123.69,	92.68, 92.68, 158.85	59.15, 59.15,
	160.02	58.01	180.85		183.1
α, β, γ (°)	90, 90, 90	90, 92.24, 90	90, 90, 90	90, 90, 90	90, 90, 120
Resolution (Å)	59.54–2.27 (2.33– 2.27)a	57.96–1.77 (1.87– 1.77)	45.21–2.04 (2.09– 2.04)	46.34–2.70 (2.77–2.70)	39.24–2.46 (2.52–2.46)
Rsym	0.097 (1.110)	0.066 (0.827)	0.087 (0.809)	0.087 (1.600)	0.075 (0.882)
CC1/2b	99.7 (58.3)	99.5 (65.9)	99.7 (64.6)	99.9 (76.2)	99.8 (79.3)
I / σI	11.1 (1.74)	12.6 (1.40)	12.2 (1.55)	19.8 (1.92)	12.2 (1.62)
Completeness (%)	99.8 (100.0)	89.0 (90.0)	97.6 (79.5)	99.8 (98.5)	99.6 (99.5)
Multiplicity	6.3 (6.5)	3.2 (3.4)	5.5 (3.1)	11.1 (11.2)	5.4 (5.5)
Refinement
Resolution (Å)	59.54–2.27	57.96–1.77	45.21–2.04	46.34–2.70	39.24–2.46
No. reflections (total / free)	39576 / 1882	24426 / 1310	33548 / 1682	19765 / 1008	13963 / 707
Rwork / Rfree	0.214 / 0.251	0.209 / 0.245	0.186 / 0.231	0.230 / 0.278	0.206 / 0.247
Number of atoms:
Protein	6288	1944	4003	3709	2078
Ligand/ion	10	140
Water	56	65	225	14	17
B-factors:
Protein	61.6	37.8	37.9	92.1	81.7
Ligand/ion	68.1	72.2
Water	48.5	47.1	39.3	40.6	63.4
All atoms	61.5	40.4	38.0	91.9	81.6
Wilson B	47.8	38.0	37.2	74.7	67.6
R.m.s. deviations:
Bond lengths (Å)	0.004	0.019	0.008	0.003	0.002
Bond angles (°)	0.657	2.130	1.166	0.722	0.494
Ramachandran distributionc (%):
Favored	97.73	94.16	97.32	96.07	95.97
Outliers	0.13	0.39	0	0.21	0.37

^aValues in parentheses are for the highest-resolution shell.

^bCC1/2 correlation coefficient as defined in Karplus & Diederichs [42] and calculated by XSCALE [41].

^cCalculated using the MolProbity server (http://molprobity.biochem.duke.edu) [76].