Table 3.
Collected SAXS data
| Data collection parameters | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Instrument | P12 at EMBL/DESY, storage ring PETRA III, Germany | ||||||||
| Beam geometry (mm2) | 0.2×0.12 | ||||||||
| Wavelength (Å) | 1.24 | ||||||||
| q-range (Å−1) | 0.0023–0.47 | ||||||||
| Exposure time (ms) | 20×50 | ||||||||
| Temperature (K) | 283 | ||||||||
| Instrument | B21 at Diamond Light Source, United kingdom | ||||||||
| Beam geometry (mm2) | 1.0×5.0 | ||||||||
| Wavelength (Å) | 1.0 | ||||||||
| q-range (Å−1) | 0.0038–0.42 | ||||||||
| Exposure time (ms) | 60×100 | ||||||||
| Temperature (K) | 283 | ||||||||
| Structural parameters | |||||||||
| Sample | Conc. (mg ml−1) |
Rg, Guinier (nm) |
Rg, Pr (nm) |
Dmax (nm) |
MMtheor (kDa) |
MMSAXS (kDa) |
MMPOROD (kDa) |
MMDAM (kDa) |
Ab initio resolution, (Å) |
| EspG1mma | 1.0 | 2.7 | 2.9 | 9.7 | 29.8 | 25.0 | 43.0 | 45.0 | 42±3 |
| 2.0 | 3.2 | 3.1 | 11.0 | 29.8 | 32.0 | 55.0 | |||
| 4.2 | 3.4 | 3.5 | 11.0 | 29.8 | 37.0 | 65.0 | |||
| 6.0 | 3.5 | 3.6 | 12.0 | 29.8 | 40.0 | 72.0 | |||
| EspG3mtu | 1.1 | 2.5 | 2.6 | 9.0 | 31.6 | 23.0 | 41.0 | 41.0 | 34±3 |
| 2.0 | 2.8 | 3.0 | 10.0 | 31.6 | 30.0 | 47.0 | |||
| 4.0 | 3.2 | 3.1 | 10.0 | 31.6 | 32.0 | 56.0 | |||
| 6.1 | 3.5 | 3.5 | 12.0 | 31.6 | 39.0 | 68.0 | |||
| EspG3mma | 1.1 | 2.3 | 2.5 | 8.0 | 32.0 | 20.0 | 39.0 | 34.0 | 23±2 |
| 2.1 | 2.3 | 2.4 | 8.0 | 32.0 | 22.0 | 35.0 | 35.0 | ||
| 4.0 | 2.3 | 2.4 | 8.0 | 32.0 | 21.0 | 40.0 | 38.0 | ||
| 6.0 | 2.3 | 2.3 | 8.0 | 32.0 | 22.0 | 44.0 | 36.0 | ||
| EspG3msm | 0.8 | 2.5 | 2.6 | 8.6 | 31.6 | 20.5 | 39.0 | 48.0 | 36±3 |
| 1.8 | 2.5 | 2.5 | 8.8 | 31.6 | 21.4 | 32.0 | 41.0 | ||
| 3.9 | 2.6 | 2.6 | 9.3 | 31.6 | 22.1 | 39.0 | 44.0 | ||
| 6.2 | 2.8 | 2.7 | 9.7 | 31.6 | 24.0 | 39.0 | 47.0 | ||
| EspG3msm Se-Met |
0.9 | 2.5 | 2.5 | 9.2 | 31.6 | 22.5 | 38.0 | 43.0 | 39±3 |
| 2.1 | 2.5 | 2.6 | 9.2 | 31.6 | 22.5 | 38.0 | |||
| 3.8 | 2.6 | 2.6 | 9.2 | 31.6 | 24.3 | 40.0 | |||
| EspG5mtu | 0.9 | 2.3 | 2.5 | 8.0 | 32.4 | 23.0 | 41.0 | 47.0 | 25±2 |
| 1.7 | 2.4 | 2.4 | 8.0 | 32.4 | 22.0 | 42.0 | |||
| 4.1 | 2.4 | 2.5 | 8.0 | 32.4 | 21.0 | 43.0 | |||
| 6.7 | 2.4 | 2.4 | 8.0 | 32.4 | 22.0 | 41.0 | |||
| EspG5mtu- PE25- PPE41 |
0.5 | 4.0 | 4.0 | 13.0 | 65.0 | 76.0 | 46.0 | 76.0 | 37±3 |
| EspG3msm- PE5-PPE4 |
1.2 | 4.0 | 4.2 | 14.2 | 95.0 | 83.0 | 52.0 | 83.0 | 38±3 |