. Author manuscript; available in PMC: 2020 Jan 18.

Published in final edited form as: J Mol Biol. 2018 Nov 10;431(2):289–307. doi: 10.1016/j.jmb.2018.11.003

Table 4.

Oligomer analysis using the crystallographic monomer and dimer structures of EspG proteins.

Sample	Template (PDB ID)	Concentration (mg ml⁻¹)	Monomeric Fraction	Dimeric fraction	Goodness-of- fit,χ²
EspG_1mma	β8-mediated dimer (4RCL)	1.0	0.94	0.06	0.80
		2.0	0.74	0.26	0.93
		4.2	0.58	0.42	1.44
		6.0	0.50	0.50	1.90
	Wing-shaped dimer (4L4W)	1.0	0.93	0.07	0.79
		2.0	0.72	0.28	0.86
		4.2	0.54	0.46	1.01
		6.0	0.43	0.57	1.27
EspG_3mtu	Wing-shaped dimer (4L4W)	1.1	0.537	0.462	0.89
		2.0	0.364	0.636	0.90
		4.0	0.113	0.887	0.95
		6.1	0.0	0.999	0.89
EspG_3mma	Wing-shaped dimer (4L4W)	1.1	0.531	0.468	0.82
		2.1	0.195	0.805	0.88
		4.0	0.000	1.000	1.05
		6.0	0.00	1.000	1.69
EspG_3msm (Native)	Wing-shaped dimer (4L4W)	0.78	0.589	0.410	0.90
		1.75	0.654	0.346	1.32
		3.94	0.575	0.425	1.35
		6.24	0.449	0.550	2.03
EspG_3msm (SetMet)	Wing-shaped dimer (4L4W)	0.90	0.70	0.30	0.92
		2.14	0.70	0.30	1.80
		3.79	0.59	0.41	2.50