Figure 4.

ERK Downregulation Precedes Caspase Activation

(A) Snapshots of two nuclei in the midline of the pupal notum showing miniCic signal (top) and Scat3 FRET signal (bottom; dark blue signal, caspase activation). Scale bars represent 10 μm.

(B) miniCic nuclear signal of the left nucleus shown in (A) (red) and FRET signal (purple).

(C) Averaged normalized profiles of miniCic nuclear intensity (red) in the midline aligned at t0 = onset of caspase activation (maximum inflexion of the FRET signal). Light colored areas are ± SEM. The averaged Scat3 FRET signal is shown in purple. Grey curve: normalized averaged miniCic signal of midline cells that do no activate caspase.

See also Video S2.