REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
rat anti E-cad	DSHB	DCAD2 concentrate; RRID:AB_528120
guinea pig anti Hid	gift of Don Hyong Ryu	N/A
mouse anti EcR	DSHB	Ag10.2 concentrate; RRID:AB_528208
rabbit anti dpERK	Cell Signaling	Phospho-p44/42 MAPK #4370; RRID:AB_2315112
chicken anti GFP	abCam	#13970; RRID:AB_300798
chicken anti mCherry	abCam	#205402; RRID:AB_2722769
rabbit anti-cleaved Dcp-1	Cell Signaling	#9578; RRID:AB_2721060
mouse anti-Argos	DSHB	Argos 85/2/16 concentrate; RRID:AB_528088
monoclonal mouse anti EGFR	Sigma	E29006 clone C-273; RRID:AB_609900
rabbit anti ERK	Cell Signaling	P44/42 MAPK #4695; RRID:AB_390779
Mouse anti alpha-tubulin	DSHB	12G10 concentrate; RRID:AB_1157911
Chemicals, Peptides, and Recombinant Proteins
Trametinib	Santa Cruz	CAS 871700-17-3
Experimental Models: Cell Lines
Drosophila S2R+ cells	François Schweisguth lab	FBrf0024118
Experimental Models: Drosophila melanogaster lines
pnr-gal4 (III)	Bloomington	BDSC_3039
w1118	Bloomington	BDSC_3605
UAS-lacZ (II)	Bloomington	BDSC_8529
UAS-diap1 (III)	Bloomington	BDSC_6657
UAS-hid ds RNA (III)	VDRC	GD 8269
UAS-rpr dsRNA (II)	VDRC	KK 101234
UAS-grim dsRNA (II)	VDRC	GD 21830
hs-flp22;ubi-ECad::GFP,UAS-mRFP; act < y+ < Gal4	L. Legoff (Marseille)	N/A
UAS-ras1V12 (III)	From [56]	N/A
cic-Cic::GFP (Bac clone) (II)	Bloomington	BDSC_42267
UAS-egfr dsRNA (II)	VDRC	KK 107130
UAS-egfr dsRNA TRiPJF01084 (III)	Bloomington	BDSC_31526
UAS-hEC::EGFR (II)	Bloomington	BDSC_58415
endo-Ecad::GFP	Bloomington	BDSC_60584
tub-GFPBantamsensor/Cyo (II)	From [17]	N/A
UAS-dsRed Bantam sponge (III)	From [57]	N/A
ubi-Ecad::RFP (II)	From [58]	N/A
UAS-pvr dsRNA (II)	VDRC	KK 105353
UAS-fak dsRNA (III)	VDRC	GD 108608
UAS-src42A dsRNA (II)	VDRC	KK 100708
UAS-src64B dsRNA (I)	VDRC	GD 35252
UAS-pi3kCAAX (I)	Bloomington	BDSC_8294
UAS-rafGOF(III)	Bloomington	BDSC_2033
tub-miniCic::mCherry (II)	This study	N/A
hs-flp22 ; endo-Ecad::GFP, tub-miniCic::mCherry ; act < cd2 < G4, UAS-eGFP	Bloomington and this study	BDSC_60584
endo-Ecad::GFP,tub-miniCic::mCherry	Bloomington and this study	BDSC_60584
UAS-scat3::myc (II)	From [29]	N/A
tub-miniCic::mcherry; UAS-ykiS111A S168A S250A	Bloomington combined with miniCic	BDSC_28817
hs-flp22; endo-Ecad::GFP, tub-gal80ts ; act < y+ < gal4 UAS-nlsRFP/TM6b	From [59]	N/A
hs-flp22; endo-Ecad::GFP, tub-gal80ts ; act < cd2 < gal4 UAS-eGFP/TM6b	This study	N/A
UAS-sSpitzCS, UAS-rasV12 (III)	Bloomington recombined with UAS-rasV12	BDSC_63134
UAS-sqhE20E21 (II) ; UAS-rasV12 (III)	From [60] combined with UAS-rasV12	N/A
UAS-argos dsRNA (II) ; UAS-rasV12 (III)	VDRC combined with UAS-rasV12	GD 47181
Oligonucleotides
miniCicF: ATCGCTGCGTGCGCGCTTAGGCGGCC GCAACATGCCAAAAAAGAAGAGAAAGGTATCCG CCTCCGGAGGGGGCGTGGTC	This study	N/A
miniCicR: AGAACCACCACCAGAACCACCACCGTA ATATTGAAAAACATCTGCC	This study	N/A
mCherryF: GGTGGTGGTTCTGGTGGTGGTTCTGT GTCCAAGGGCGAAGAGGAC	This study	N/A
mCherryR: GCGCGATGCCGACTGAGTAGGTCTAG ATTATTTATACAGCTCGTCCAT	This study	N/A
Recombinant DNA
pCaSperR4-tubp-Gal80 plasmid	Addgene	#17748
Software and Algorithms
MATLAB with Image processing toolbox and Statistics and Machine learning toolbox	Mathworks	https://fr.mathworks.com/
MatPIV	Department of Mathematics, University of Oslo	https://www.mn.uio.no/math/english/people/aca/jks/matpiv/
Fiji (ImageJ)	Fiji	https://fiji.sc/
Mypic Zen autofocus macro	Jan Ellenberg	https://git.embl.de/grp-ellenberg/mypic
Adaptive local z-projection macro (MATLAB)	This study	Supplementary information (Methods S1)
PIV algorithm (MATLAB)	[61]	N/A
Graphpad Prism8	Graphpad	https://www.graphpad.com/
Packing analyzer (now tissue miner in Fiji)	[62], Benoit Aigouy	https://idisk-srv1.mpi-cbg.de/∼eaton/