Figure	Genotype	Heat shock 37°C	Stage	n
1B	UAS-lacZ/+ ; pnr-gal4/+	N/A	adult	35
1B	pnr-gal4/UAS-diap1	N/A	adult	19
1B	pnr-gal4/UAS-hid dsRNA	N/A	adult	26
1B	UAS-rpr dsRNA/+ ; pnr-gal4/+	N/A	adult	25
1C	hs-flp22/+; ubi-Ecad::GFP,UASmRFP/+; act < y+ < G4/+	12 min, 3 days ACI	Pupal notum (16h APF)	4
1C	hs-flp22/+ ; ubi-Ecad::GFP, UASmRFP/+; act < y+ < G4/UAS-hid dsRNA	12 min, 3 days ACI	Pupal notum (16h APF)	5
1C	hs-flp22/+ ; ubi-Ecad::GFP, UASmRFP/UAS-rpr dsRNA; act < y+ < G4/+	12 min, 3 days ACI	Pupal notum (16h APF)	2
1E	w1118	N/A	Pupal notum (30h APF)	7
1F	hs-flp22/+; endo-Ecad::GFP, tub-gal80ts/+ ; act < y+ < gal4, UAS-nlsRFP/UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	4
2A	cic-Cic::GFP (Bac clone) (II)	N/A	Pupal notum (30h APF)	7
2B	UAS-lacZ/+ ; pnr-gal4/+	N/A	adult	35
2B	UAS-egfr dsRNA/+ ; pnr-gal4/+	N/A	adult	23
2B	pnr-gal4/UAS-hid dsRNA	N/A	adult	26
2B	UAS-egfr dsRNA/+ ; UAS-hid dsRNA/pnr-gal4	N/A	adult	20
2C	hs-flp22/+ ; ubi-Ecad::GFP, UASmRFP/UAS-egfr dsRNA; act < y+ < G4/+	12 min, 3 days ACI	Pupal notum (16h APF)	3
2C	hs-flp22/+ ; ubi-Ecad::GFP, UASmRFP/UAS-hegfr::GFP; act < y+ < G4/+	12 min, 3 days ACI	Pupal notum (16h APF)	3
S1A	endo-Ecad::GFP	N/A	Pupal notum (30h APF)	3
S1B	tub-GFPBantamsensor/ubi-Ecad::RFP	N/A	Pupal notum (16h APF)	3
S1C	pnr-gal4/UAS-dsRed Bantam sponge	N/A	Pupal notum (30h APF)	4
S1D,E	hs-flp22/+ ; ubi-Ecad::GFP, UASmRFP/+; act < y+ < G4/UAS-dsRed Bantam sponge	12 min, 3 days ACI	Pupal notum (16h APF)	2
S1F	w1118	N/A	Pupal notum (30h APF)	5
S2A	UAS-pvr dsRNA/+ ; pnr-gal4/+	N/A	adult	10
S2A	UAS-fak dsRNA/pnr-gal4	N/A	adult	10
S2A	UAS-src42A dsRNA/+ ; pnr-gal4/+	N/A	adult	13
S2A	UAS-src64B dsRNA/+ ; ;pnr-gal4/+	N/A	adult	13
S2B	hs-flp22/UAS-pi3kCAAX; ubi-Ecad::GFP, UASmRFP/+; act < y+ < G4/+	12 min, 3 days ACI	Pupal notum (16h APF)	2
S2C	hs-flp22/+;ubi-Ecad::GFP,UASmRFP/+; act < y+ < G4/UAS-rafGOF	12 min, 2 days ACI	Pupal notum (16h APF)	2
3B	tub-miniCic::mCherry (II)	N/A	Wing imaginal disc and eye imaginal disc from L3 wandering stage	10
3C	hs-flp22/+ ; endo-Ecad::GFP, tub-miniCic::mCherry/UAS-egfr dsRNA ; act < cd2 < G4, UAS-eGFP/+	12 min, 2 days ACI	Pupal notum (20h APF)	6
3C	hs-flp22/+ ; endo-Ecad::GFP, tub-miniCic::mCherry/UAS-hegfr::GFP; act < cd2 < G4, UAS-eGFP/+	12 min, 2 days ACI	Pupal notum (20h APF)	4
3E	tub-miniCic::mCherry (II)	N/A	Haemocytes from L3 wandering stage larvae	2 cultures
3F	endo-Ecad::GFP, tub-miniCic::mCherry	N/A	Pupal notum (16h APF)	3
S3A	tub-miniCic::mCherry	N/A	Embryos stage 5 to 7	56
S3B	endo-Ecad::GFP, tub-miniCic::mCherry	N/A	Pupal notum (16h APF)	1
4A-C	tub-miniCic::mCherry/UAS-Scat3 ; pnr-gal4/+	N/A	Pupal notum (16h APF)	2
5A-C	endo-Ecad::GFP, tub-miniCic::mCherry	N/A	Pupal notum (20h APF)	3
5E-I	endo-Ecad::GFP, tub-miniCic::mCherry	N/A	Pupal notum (20h APF)	3
5J-L	endo-Ecad::GFP, tub-miniCic::mCherry	N/A	Pupal notum (30h APF)	4
S4A-C	hs-flp22/+ ; endo-Ecad::GFP, tub-gal80ts/tub-miniCic::mCherry; act < cd2 < G4, UAS-eGFP/ UAS-ykiS111A S168A S250A	40 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	10
6A,B	hs-flp22/+; endo-Ecad::GFP, tub-gal80ts/tub-miniCic::mCherry ; act < y+ < gal4, UAS-nlsRFP/UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (20h APF)	4
6C-F	hs-flp22/+; endo-Ecad::GFP, tub-gal80ts/+ ; act < y+ < gal4, UAS-nlsRFP/ UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (20h APF)	4
6C-F	hs-flp22/+; endo-Ecad::GFP, tub-gal80ts/+ ; act < y+ < gal4, UAS-nlsRFP/ UAS-rasV12, UAS-sSpitzCS	30 min, 1 week 18°C, 24h 29°C	Pupal notum (20h APF)	4
6C-F	hs-flp22/+; endo-Ecad::GFP, tub-gal80ts/UAS-sqhE20E21 ; act < y+ < gal4, UAS-nlsRFP/ UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (20h APF)	3
S5A	hs-flp22/+;endo-Ecad::GFP,tub-gal80ts/ tub-miniCic::mCherry ; act < y+ < gal4, UAS-nlsRFP/UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	4
S5B	hs-flp22/+;endo-Ecad::GFP,tub-gal80ts/ tub-miniCic::mCherry ; act < y+ < gal4, UAS-nlsRFP/UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	4
S5B	hs-flp22/+;endo-Ecad::GFP,tub-gal80ts/ tub-miniCic::mCherry; act < y+ < gal4, UAS-nlsRFP/ UAS-rasV12, UAS-sSpitzCS	30 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	6
S5C	hs-flp22/+;endo-Ecad::GFP,tub-gal80ts/+; act < y+ < gal4, UAS-nlsRFP/ UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	3
S5C	hs-flp22/+;endo-Ecad::GFP,tub-gal80ts/UAS-argos dsRNA; act < y+ < gal4,UAS-nlsRFP/UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (30h APF)	3
S5D,E	hs-flp22/+;endo-Ecad::GFP,tub-gal80ts/UAS-argos dsRNA; act < y+ < gal4,UAS-nlsRFP/UAS-rasV12	30 min, 1 week 18°C, 24h 29°C	Pupal notum (20h APF)	2
S6A-C	hs-flp22/+ ; ubi-Ecad::GFP, UASmRFP/UAS-egfr dsRNA; act < y+ < G4/+	12 min, 3 days ACI	Pupal notum (16h APF)	3
S6D	endo-Ecad::GFP	N/A	Pupal notum (25h APF)	5