Skip to main content
. 2019 Jan 7;29(1):35–50.e4. doi: 10.1016/j.cub.2018.11.009

Figure 1.

Figure 1

The Forelimb Position Is Already Determined by the End of Gastrulation

(A) LPM rotation procedure in a stage 11 chicken embryo. FL, Forelimb; IL, Interlimb.

(B–D) Fgf10 (B and C) and Tbx5 (D) expression 48 hr after LPM rotation, showing complete (B; n = 3/23 embryos, red brackets) or partial (C and D; n = 12/23 embryos, red arrowhead) displacement of the forelimb bud.

(E) Chicken embryo grafted with memGFP transgenic quail somatopleure (green) showing a posterior displacement of the forelimb upon rotation (n = 3/3 embryos).

(F–I) Transverse sections of quail-chick chimera at the forelimb (F and G) and interlimb (H and I) levels, stained with phalloidin (red), GFP antibody (green), and DAPI (blue). (G) and (I) are higher magnification of (F) and (H), respectively. NT, neural tube; S, somite; IM, intermediate mesoderm; Som, somatopleure.

Scale bars are 500 μm in (B)–(E) and 100 μm in (F)–(I).