REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit polyclonal anti-GFP | Torrey Pines Biolabs | Cat#TP-401; RRID: AB_10013661 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 | Thermo Fisher Scientific | Cat#A32731; RRID:AB_2633280 |
Chemicals, Peptides, and Recombinant Proteins | ||
Alexa Fluor 555 Phalloidin | Thermo Fisher Scientific | Cat#A34055 |
Fluoromount-G, with DAPI | Thermo Fisher Scientific | Cat#00-4959-52 |
RA | Sigma-Aldrich | Cat#R2625 |
AGN 193109 | Tocris | Cat#5758 |
Alcian Blue 8GX | Sigma-Aldrich | Cat#A9186 |
Alizarin Red S | Sigma-Aldrich | Cat#A5533 |
Experimental Models: Organisms/Strains | ||
Fertilized chicken eggs | EARL Morizeau | N/A |
Fertilized ostrich eggs | SARL Le père Louis | N/A |
Fertilized zebra finch eggs | Laboratory of Marie Manceau | N/A |
Transgenic quail: hUbC:memGFP | This paper | N/A |
Transgenic quail: hUbC:mEOS2FP | This paper | N/A |
Oligonucleotides | ||
Primer Hoxb4 dn Forward: AATAAATCTAGAATGGCCATGAGCT CGTTTTTGATCAACTCC |
This paper | N/A |
Primer Hoxb4 dn Reverse: TTTTGGCGCGCCTCAGATTTTGATCTGGC | This paper | N/A |
Primer Hoxb4 C-terminal Forward: CCAGAACAGGAGGATGAAATGG | This paper | N/A |
Primer Hoxb4 C-terminal Reverse: TTATAGGCTGCTGGCTGGTCC | This paper | N/A |
Primer Hoxc9 dn Forward: TTTGCTAGCATGTCGGCTTCTGGCCCC ATAAGC |
This paper | N/A |
Primer Hoxc9 dn Reverse: TTTGGCGCGCCTCAGATTTTGACTTGGC | This paper | N/A |
Primer cCyp26a1 Forward: ATCCTGCTGGGCTTCCAGCCC | This paper | N/A |
Primer cCyp26a1 Reverse: GGCCGCTGAAACCTATGAATTTGG | This paper | N/A |
Recombinant DNA | ||
pCAGGS-H2b-RFP | Laboratory of S. Tajbakhsh | N/A |
pFlox-pA-EGFP | [47] | N/A |
pCX-Cre | [47] | N/A |
pCAG-GFP | [48] | Addgene Cat#11150 |
pCAGGS-cHoxb4-IRES2-Venus | This paper | N/A |
pCAGGS-cHoxb4 dn-IRES2-Venus | This paper | N/A |
pCAGGS-cHoxb7-IRES2-Venus | This paper | N/A |
pCAGGS-cHoxb9-IRES2-Venus | This paper | N/A |
pCAGGS-cHoxc9 dn-IRES2-Venus | This paper | N/A |
pBS-Hoxb4 | [28] | N/A |
pBSKS-Hoxb7 | [28] | N/A |
pGEMT easy-Hoxb9 | [28] | N/A |
pSLAX-cTbx5 | Laboratory of C. Tabin | N/A |
pBSSK-cFgf10 | Laboratory of C. Tabin | N/A |
pBSSK-cShh | Laboratory of C. Tabin | N/A |
pGEMT easy-cCyp26a1 | This paper | N/A |
pGEMT easy-cHoxb4Cter | This paper | N/A |
Software and Algorithms | ||
ImageJ | [49] | RRID:SCR_003070 |
GraphPad Prism 6 | GraphPad Software | RRID:SCR_002798 |