Skip to main content
. 2019 Jan 7;29(1):35–50.e4. doi: 10.1016/j.cub.2018.11.009
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit polyclonal anti-GFP Torrey Pines Biolabs Cat#TP-401; RRID: AB_10013661
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 Thermo Fisher Scientific Cat#A32731; RRID:AB_2633280

Chemicals, Peptides, and Recombinant Proteins

Alexa Fluor 555 Phalloidin Thermo Fisher Scientific Cat#A34055
Fluoromount-G, with DAPI Thermo Fisher Scientific Cat#00-4959-52
RA Sigma-Aldrich Cat#R2625
AGN 193109 Tocris Cat#5758
Alcian Blue 8GX Sigma-Aldrich Cat#A9186
Alizarin Red S Sigma-Aldrich Cat#A5533

Experimental Models: Organisms/Strains

Fertilized chicken eggs EARL Morizeau N/A
Fertilized ostrich eggs SARL Le père Louis N/A
Fertilized zebra finch eggs Laboratory of Marie Manceau N/A
Transgenic quail: hUbC:memGFP This paper N/A
Transgenic quail: hUbC:mEOS2FP This paper N/A

Oligonucleotides

Primer Hoxb4 dn Forward: AATAAATCTAGAATGGCCATGAGCT
CGTTTTTGATCAACTCC
This paper N/A
Primer Hoxb4 dn Reverse: TTTTGGCGCGCCTCAGATTTTGATCTGGC This paper N/A
Primer Hoxb4 C-terminal Forward: CCAGAACAGGAGGATGAAATGG This paper N/A
Primer Hoxb4 C-terminal Reverse: TTATAGGCTGCTGGCTGGTCC This paper N/A
Primer Hoxc9 dn Forward: TTTGCTAGCATGTCGGCTTCTGGCCCC
ATAAGC
This paper N/A
Primer Hoxc9 dn Reverse: TTTGGCGCGCCTCAGATTTTGACTTGGC This paper N/A
Primer cCyp26a1 Forward: ATCCTGCTGGGCTTCCAGCCC This paper N/A
Primer cCyp26a1 Reverse: GGCCGCTGAAACCTATGAATTTGG This paper N/A

Recombinant DNA

pCAGGS-H2b-RFP Laboratory of S. Tajbakhsh N/A
pFlox-pA-EGFP [47] N/A
pCX-Cre [47] N/A
pCAG-GFP [48] Addgene Cat#11150
pCAGGS-cHoxb4-IRES2-Venus This paper N/A
pCAGGS-cHoxb4 dn-IRES2-Venus This paper N/A
pCAGGS-cHoxb7-IRES2-Venus This paper N/A
pCAGGS-cHoxb9-IRES2-Venus This paper N/A
pCAGGS-cHoxc9 dn-IRES2-Venus This paper N/A
pBS-Hoxb4 [28] N/A
pBSKS-Hoxb7 [28] N/A
pGEMT easy-Hoxb9 [28] N/A
pSLAX-cTbx5 Laboratory of C. Tabin N/A
pBSSK-cFgf10 Laboratory of C. Tabin N/A
pBSSK-cShh Laboratory of C. Tabin N/A
pGEMT easy-cCyp26a1 This paper N/A
pGEMT easy-cHoxb4Cter This paper N/A

Software and Algorithms

ImageJ [49] RRID:SCR_003070
GraphPad Prism 6 GraphPad Software RRID:SCR_002798