Figure 1.
CD169 Limits Retrovirus Dissemination from pLN to Spleen and Is Required for Efficient FrMLV Infection
(A) Scheme indicating a possible path of virus dissemination from popliteal lymph node (pLN) to blood and spleen after subcutaneous (s.c.) footpad administration of luciferase expressing FrMLV.
(B–D) The indicated organs and plasma were harvested 1 hr after virus administration as in (A). The graphs show viral loads measured as relative luciferase units at indicated locations after performing highly sensitive virus load assay in which plasma (n = 5), pLN (n = 7), and splenocyte (n = 5) cell suspensions were incubated with DFJ8 cells for 36–48 hr before measuring luciferase activity.
(E and F) FrMLV-infected cells 5 dpi (s.c., 4 × 105 IU) at pLN (n = 10) and spleen (n = 5) in B6 and CD169−/− mice.
(G) A model depicting FrMLV dissemination and subsequent levels of infection 5 dpi from pLN to blood and the spleen following subcutaneous challenge in B6 and CD169−/− mice to show the infection-promoting role of CD169.
p values derived from non-parametric Mann-Whitney test; mean values denoted by horizontal line.