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Principal component analysis based on OTUs of the napA, narG, nirS and nosZ libraries (A–D). Three replicate DNA libraries are shown (designated DNA.1, DNA.2 and DNA.3). For the cDNA, three libraries designated cDNA1, cDNA2, and cDNA3 were obtained, representing the three sampling times (after 1, 2, and 4 h of incubation). For each of these, three replicate samples were analyzed, designated “0.1,” “0.2,” and “0.3”.
