Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 8;12:514. doi: 10.3389/fncel.2018.00514

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Miyazaki, Lisman and Ross.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Schematic of modified apparatus including both LEDs and lasers. The box at the top contains four lasers at the indicated wavelengths. The laser outputs, which can be separately controlled, are combined and focused onto either a 50 μm or 200 μm diameter fiber optic. The face of the fiber is focused with a lens and the objective onto a region of the slice, after 30% of the light is reflected off a mirror in the microscope body and a special DM in a standard filter cube (see Figure 1B for a typical filter set). The LED outputs are combined and 70% of the light passes through to preparation. Software selects combinations of LEDs and lasers to excite the indicator dyes in the cell of interest. The CCD camera (RedShirtImaging, NeuroCCD-SMQ) detects the fluorescence changes with frames synchronized with the laser or LED pulses.