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FgShy1 induction in mung bean liquid medium with addition of 1 mM SA. F. graminearum strain 46422 was grown overnight and then incubated additional 2 h with or without 1 mM SA. Samples were collected for RNA isolation, cDNA synthesis and gene expression analysis. Fungal β-tubulin expression was measured and used as an internal control for transcript normalization. Gene expression levels for cultures grown in the presence of SA were determined relative to controls grown without SA.
