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. 2019 Jan 8;11:469. doi: 10.3389/fnmol.2018.00469

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Copyright © 2019 Wang, Zhu, Long, Pan, Xiong, Fang, Hill, Lai, Yuan and Sha.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of MCU via the pharmacological inhibitor Ru360 or siMCU attenuated noise-induced increases in cleaved caspase 9 (CC9) in OHCs of the basal turn. (A) Representative images show an increase in immunoreactivity for CC9 (red) in OHCs stained with phalloidin 1 h after completion of the exposure (panel 2) compared to control mice without exposure (panel 1). Treatment with Ru360 attenuated noise-induced CC9 in OHCs (panel 3). Images were taken from the region of the surface preparations corresponding to sensitivity to 22–32 kHz using a Leica SP5 confocal microscope; scale bar = 10 μm. (B) Quantification of CC9 in OHCs confirmed a significant increase after noise exposure and attenuation of this increase with Ru360 treatment; n = 4 per group with one cochlea used per mouse. Data are presented as means + SD, **p < 0.01. (C) Representative images show that pretreatment with siMCU decreases immunoreactivity for CC9 (red) in OHCs stained with phalloidin (green) 1 h after completion of the exposure compared to siControl treatment. Images were taken from the region of the surface preparations corresponding to sensitivity to 22–32 kHz using a Zeiss confocal microscope; scale bar = 10 μm. (D) Pretreatment with siMCU also significantly reduced noise-increased immunolabeling for CC9 in OHCs compared to mice exposed to scrambled siRNA (siControl). Data are presented as means + SD, n = 4 per group with one cochlea used per mouse, *p < 0.05.