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. 2019 Jan 8;9:1158. doi: 10.3389/fneur.2018.01158

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Copyright © 2019 Yao, Zhang, Zhang, Guo, Li, Xiao, Liu, Shen and Ni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Further validation of the potential protein biomarkers for AD by Western blot analyses. (A) The concentration of VLDLR was decreased significantly in AD samples, with **p < 0.01 vs. control samples. (B) The concentration of TIMP1 was increased significantly in AD samples, with ***p < 0.001 vs. control samples. The expression level of protein was normalized by the mean of the controls (n = 5), with each bar representing SEM. The upper images of Western blot analysis correspond to the lower histograms of semi-quantification. The statistical results of the data were show as *p < 0.05, **p < 0.01, ***p < 0.001. (C) A loading control is presented aiming to verify the normalization of protein amounts.