Figure 1.

Loss of JAK1 in NKp46⁺ cells leads to an almost complete absence of peripheral NK cells. (A) Frequency (left panel) and total numbers (right panel) of Lin⁻(CD3⁻CD19⁻Ly-6G⁻Ter119⁻) CD122⁺ NK cells in the bone marrow were assessed by flow cytometry. (B) Bone marrow Lin⁻CD122⁺ cells were further divided into NK precursors (NKPs: NKp46⁻NK1.1⁻), immature NK cells (iNKs: NKp46⁻NK1.1⁺), and mature NK cells (mNKs: NKp46⁺NK1.1⁺). (C) Frequency of CD3⁻NKp46⁺ NK cells in the spleen was assessed by flow cytometry and representative plots are shown. (D,E) Frequency (left panel) and total numbers (right panel) of CD3⁻NKp46⁺ NK cells in the (D) spleen and (E) blood were assessed by flow cytometry. (F) Frequency of conventional NK cells (CD3⁻NK1.1⁺NKp46⁺CD49b⁺, left panel) and ILC1 cells (CD3⁻NK1.1⁺NKp46⁺CD49a⁺, right panel) was analyzed in the liver of Jak1^WT, Jak1^fl/+Ncr1Cre, and Jak1^fl/flNcr1Cre mice by flow cytometry. (A,B,D–F) Bar graphs represent mean ± SEM of 1–2 independent experiments; n = 3–11. *p < 0.05, **p < 0.01, ***p < 0.001.