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. 2019 Jan 8;9:3108. doi: 10.3389/fimmu.2018.03108

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Copyright © 2019 Witalisz-Siepracka, Klein, Prinz, Leidenfrost, Schabbauer, Dohnal and Sexl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JAK2 is dispensable for NK cell survival and maturation. (A) Frequency (left panel) and total numbers (right panel) of CD3⁻NKp46⁺ NK cells in the spleens of Jak2^WT and Jak2^fl/flNcr1Cre mice were assessed by flow cytometry. (B) Splenic CD3⁻NKp46⁺ NK cells were analyzed for expression of CD27 and CD11b by flow cytometry. Frequency of cells in each maturation stage is show: DN (CD27⁻CD11b⁻), CD27 (CD27⁺CD11b⁻), DP (CD27⁺CD11b⁺), CD11b (CD27⁻CD11b⁺). (C) The expression of JAK2 and β-actin was analyzed by Western blot in NK cells upon 6 days of expansion in IL-2. Scans of full blots are available in Supplementary Material. (A,B) Bar graphs represent mean ± SEM of 2–3 independent experiments; n = 5–12.