Figure 4.

Loss of one allele of Jak1 impairs NK cell activity. (A) NK cells were MACS-purified from spleens and cultured in IL-2 for 7 days. The numbers of living CD3⁻NK1.1⁺NKp46⁺ cells were assessed every 2–3 days. The symbols and the error bars present mean ± SEM of 2–4 biological replicates. (B) The expression of JAK1, Perforin and β-actin was analyzed in expanded NK cells from two independent experiments by Western blot. Scans of full blots are available in Supplementary Material. (C) Expanded NK cells from Jak1^WT and Jak1^fl/+Ncr1Cr mice were mixed with CFSE-stained YAC-1 (left panel) or RMA-Rae1 (right panel) target cells at indicated effector target ratios. The specific lysis was assessed by flow cytometry. For YAC-1 a representative graph of one of two independent experiments is shown. The symbols and the error bars present mean ± SEM of 2 technical replicates. For RMA-Rae1 a mean out of two independent experiments is shown. The symbols and the error bars present mean ± SEM of 2–3 biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001.