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. 2019 Jan 8;9:1539. doi: 10.3389/fphar.2018.01539

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Copyright © 2019 Fu, Xi, Wang, Zhai, Wang, Gong, Xu, Yang, Qiu, Wang, Lu and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of CHB-II-F on protein expression of caspase-3, caspase-8, caspase-9, Bax and Bcl-2 in tumor tissue of the chemotherapy-treated H₂₂ hepatocellular carcinoma mouse model. (A) Cleaved-caspase 3 protein level; (B) Caspase 3 protein level; (C) Cleaved-caspase 8 protein level; (D) Caspase 8 protein level; (E) Cleaved-caspase 9 protein level; (F) Caspase 9 protein level; (G) Bcl-2 protein level; (H) Bax protein level. Data were presented as the mean ± SD from 6 mice. Statistical analysis: ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 compared with the saline group; P < 0.05, P < 0.01 compared with the YZXJC group. (The same loading control was used for uncleaved and cleaved versions of the same protein, and for Bcl-2 and Bax for ease of comparison).

Inline graphic — Effects of CHB-II-F on protein expression of caspase-3, caspase-8, caspase-9, Bax and Bcl-2 in tumor tissue of the chemotherapy-treated H₂₂ hepatocellular carcinoma mouse model. (A) Cleaved-caspase 3 protein level; (B) Caspase 3 protein level; (C) Cleaved-caspase 8 protein level; (D) Caspase 8 protein level; (E) Cleaved-caspase 9 protein level; (F) Caspase 9 protein level; (G) Bcl-2 protein level; (H) Bax protein level. Data were presented as the mean ± SD from 6 mice. Statistical analysis: ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 compared with the saline group; P < 0.05, P < 0.01 compared with the YZXJC group. (The same loading control was used for uncleaved and cleaved versions of the same protein, and for Bcl-2 and Bax for ease of comparison).