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. 2019 Jan 8;12:988. doi: 10.3389/fnins.2018.00988

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Copyright © 2019 Ryan, Zarruk, Lößlein and David.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence labeling for ferritin in macrophage/microglia. Double immunofluorescence labeling show that ferritin+/CD11b+ macrophage/microglia are located mainly in the lesion (L) along the lesion border in both wildtype (A–C) and CpKO (D–F) mice 72 h after pMCAO. Dotted line demarcates the lesion boundary. The merged images (C,F) show CD11b, ferritin and DAPI nuclear staining. Note that the number of CD11b+/ferritin+ doubled labeled cells are not significantly different in the two genotypes (G). Scale bar = 100 μm.