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. 2019 Jan 8;12:988. doi: 10.3389/fnins.2018.00988

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Copyright © 2019 Ryan, Zarruk, Lößlein and David.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in protein oxidation detected by 3-nitrotyrosine labeling. Tissue sections of wildtype (A) and CpKO (B) mice showing immunohistochemical staining for 3-NT. The 3-NT staining is seen as a brown reaction product. (C) Graph shows that the number of 3-NT+ cells shows a trend to be higher in CpKO compared to wildtype mice but does not reach statistical significance. The tissue sections were counterstained with Mayer’s hemalum which labels the cell nuclei blue. n = 4; scale bar = 100 μm.