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. 2019 Jan 8;11:470. doi: 10.3389/fnmol.2018.00470

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Copyright © 2019 Mendsaikhan, Takeuchi, Walker and Tooyama.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of expression of key differentially expressed genes identified by microarray. (A) Real time PCR measurement of expression of thioredoxin-interacting protein (TXNIP) in different clones. Results are combined of three independent experiments. Data analyzed by one-way ANOVA with Tukey's post-hoc test of significance. Results indicate level of significance. *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Western blot showing relative levels of TXNIP protein in different clones compared to expression of FtMt and β-actin. ns: not significantly different (C) Real time PCR measurement of expression of thioredoxin (TXN) in different clones. Results are combined of three independent experiments. Data show no significant differences between expression levels of different clones. (D) Real time PCR measurement of expression of microsomal glutathione transferase (MGST)−1 in different clones. Results are combined of three independent experiments. Data analyzed by one-way ANOVA with Tukey's post-hoc test of significance. Results indicate level of significance. *p < 0.05; **p < 0.01. (E) Real time PCR measurement of expression of growth differentiation factor-15 (GDF-15) in different clones. Results are combined of three independent experiments. Data analyzed by one-way ANOVA with Tukey post-hoc test of significance. Results indicate level of significance. **p < 0.01. (F) Real time PCR measurement of expression of calcitonin gene related peptide alpha (CALCA) in different clones. Results are combined of three independent experiments. Data analyzed by one-way ANOVA with Tukey's post-hoc test of significance. Results indicate level of significance. **p < 0.01. (G) Real time PCR measurement of expression of secretogranin-2/chromogranin C (SCG-2) in different clones. Results are combined of three independent experiments. Data analyzed by one-way ANOVA with Tukey's post-hoc test of significance. Results indicate level of significance. *p < 0.05.