Figure 4.

Heat map representing the inhibitory effect of acetic acid and clotrimazole, fluconazole, miconazole, and tioconazole against C. albicans, C. glabrata BG2 and C. glabrata CBS138 in MM medium (at pH 4). To assess an eventual synergistic effect of acetic acids with the different azoles in determining growth inhibition of Candida spp., growth of the different strains in MM medium (at pH4) in the presence of different concentrations of acetic acid and of the different azoles was assessed based on the OD600nm after 24 h of incubation in the presence of the acids, as detailed in Materials and Methods. Synergism between acetic acid and an azole was only considered when growth inhibition achieved in the presence of the acid and of the azole was, at least, 50% higher the inhibition observed in the presence of the azole alone or of the acid alone. Conditions where the acid and an azole were found to synergize are represented by an arrow. Details on the results obtained in those conditions where azoles and acetic acid were found to synergize are provided in Supplementary Figure S7 (^*p-value below 0.05; ^**p-value below 0.01; ^***p-value below 0.001; ^****p-value below 0.0001).