Figure 2.

Effects of B. amyloliquefaciens on DSS-induced Histopathological Damage and Apoptosis. (Upper) HE staining of colon morphology (40×). Arrow, obvious edema. (Middle) ultrastructural observation of colon (transmission electron microscopy, 10,000×). Arrow, mitochondria. (Lower) TUNEL staining (yellow) for assessment of apoptosis (400×), nuclei were stained with DAPI (blue). Control, mice were untreated for 7 days and then orally gavaged PBS for 7 days; DSS, mice were supplemented with 3.5% DSS for 7 days and then orally gavaged PBS for 7 days; BaL, mice were supplemented with 3.5% DSS for 7 days and then orally gavaged B. amyloliquefaciens (Ba, 9.0 × 10⁸ CFU/kg in 200 μL of PBS/mouse/day) for 7 days; BaH, mice were supplemented with 3.5% DSS for 7 days and then orally gavaged B. amyloliquefaciens (Ba, 10.0 × 10⁸ CFU/kg in 200 μL of PBS/mouse/day).