Figure 5.

Biofunction Prediction of Microbial Communities and expression of proteins involved in metabolic signaling pathway in colonic tissue. KEEG pathway annotations in level 1 (A), level 2 (B), and level 3 (C). Protein expression of mTORC1 (D,E), AMPK (D,F) and GCN2 (D,G). AMPK, AMP-activated protein kinase; GCN2, general amino acid control non-derepressible 2; mTORC1, mammalian target of rapamycin complex 1. Control, mice were untreated for 7 days and then orally gavaged PBS for 7 days; DSS, mice were supplemented with 3.5% DSS for 7 days and then orally gavaged PBS for 7 days; BaL, mice were supplemented with 3.5% DSS for 7 days and then orally gavaged B. amyloliquefaciens (Ba, 9.0 × 10⁸ CFU/kg in 200 μL of PBS/mouse/day) for 7 days; BaH, mice were supplemented with 3.5% DSS for 7 days and then orally gavaged B. amyloliquefaciens (Ba, 10.0 × 10⁸ CFU/kg in 200 μL of PBS/mouse/day). Values are expressed as mean ± SEM, n = 6. ^a,b,cMeans of the bars with different letters were significantly different among groups (P < 0.05).