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. 2019 Jan 8;9:1915. doi: 10.3389/fpls.2018.01915

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Copyright © 2019 Pérez-Pérez, Carneros, Berenguer, Solís, Bárány, Pintos, Gómez-Garay, Risueño and Testillano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cellular organization of main stages of somatic embryogenesis. Micrographs of semithin sections stained by Toluidine blue. (A–C) Panoramic views of cluster of proembryogenic masses (A), torpedo embryo (B) and cotyledonary embryo (C). (D–G) Details at higher magnification of representative regions of (A–C), as indicated by arrows and squares. (D,E) PEMs showing clusters of embryogenic cells at the periphery (D), as indicated by thin arrow in (A), and inside (D), as indicated by open arrow in (A). (F) Torpedo embryo. (G) Cotyledonary embryo. Bars in (A,B): 0.5 mm, in (C): 1 mm, in (D,E): 20 μm.