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. 2019 Jan 8;9:1915. doi: 10.3389/fpls.2018.01915

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Copyright © 2019 Pérez-Pérez, Carneros, Berenguer, Solís, Bárány, Pintos, Gómez-Garay, Risueño and Testillano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temporal profiles of esterified/de-esterified pectins and AGPs during somatic embryogenesis. Equal amounts of extracts were dotted for each developmental stage: proembryogenic masses (PEMs), heart-torpedo embryos, and cotyledonary embryos. (A) Immuno dot blot assays with JIM7 antibody (esterified pectins), JIM5 antibody (de-esterified pectins), and LM19 antibody (de-esterified pectins). (B) Immuno dot blot assays with LM6 and LM2 antibodies for specific AGP epitopes. (C) Dot blots stained by β-glucosyl-Yariv reagent, that binds to AGPs, and β-mannosyl-Yariv reagent, which does not bind AGPs; from left to right: PEMs, heart-torpedo embryos, cotyledonary embryos, PBS (negative control) and Gum arabic (positive control). Strip of β-Gluc-Yariv shows increasing color intensity (indicating increase of AGPs) during progression of somatic embryogenesis. Strip control stained with β-Man-Yariv does not provide color signal in any dot.