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. 2019 Jan 8;9:1915. doi: 10.3389/fpls.2018.01915

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Copyright © 2019 Pérez-Pérez, Carneros, Berenguer, Solís, Bárány, Pintos, Gómez-Garay, Risueño and Testillano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence of esterified and de-esterified pectins at advanced stages of somatic embryogenesis. Confocal microscopy images of merged fluorescence signals from pectins (green) and DAPI-stained nuclei (blue). (A,C,E) Immunofluorescence for esterified pectins with JIM7 antibodies (A), and LM20 antibodies (C,E). (B,D,F) Immunofluorescence for de-esterified pectins with JIM5 antibodies (B), and LM19 antibodies (D,F). (A–D) Torpedo embryo, region similar to Figure 2F. (E,F) Cotyledonary embryo, region similar to Figure 2G. Insets show higher magnification images of epidermis and subjacent tissues. Arrows point to epidermis cells. All pectin antibodies localize in cell walls. Bars represent 20 μm.