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. 2019 Jan 8;9:1915. doi: 10.3389/fpls.2018.01915

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Copyright © 2019 Pérez-Pérez, Carneros, Berenguer, Solís, Bárány, Pintos, Gómez-Garay, Risueño and Testillano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescence of AGP epitopes recognized by LM6 and LM2 antibodies during somatic embryogenesis. Confocal microscopy images of merged fluorescence signals from pectins (green) and DAPI-stained nuclei (blue). (A–C) Immunofluorescence for AGP epitopes with LM6 antibodies. (D–F) Immunofluorescence for AGP epitopes with LM2 antibodies. (A,D) Proembryogenic masses, region similar to Figure 2D. (B,E) Torpedo embryo, region similar to Figure 2F. (C,F) Cotyledonary embryo, region similar to Figure 2G. Inset show higher magnification image illustrating labeling in cell walls and small cytoplasmic spots. Bars represent 20 μm.