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. 2019 Jan 8;9:3258. doi: 10.3389/fmicb.2018.03258

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Copyright © 2019 Zhao, Sun, Li, Sun, Yue, Wei, Wei and Jia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection of RSMV in the leafhoppers Nephotettix cincticeps and Nephotettix virescens at 20 days padp. (A,C) Leafhoppers fed on healthy plants. (B,D) Leafhoppers fed on RSMV-infected plants. The internal organs (panel I), central nervous system (panel II) and salivary glands (panel III) of leafhoppers were stained for viral antigen with N–rhodamine (red) and for actin with phalloidin–Alexa Fluor 488 carboxylic acid (green) and examined by confocal microscopy. Amg, anterior midgut; Cns, central nervous system; Es, esophagus; Fc, filter chamber; Hg, hindgut; Mmg, middle midgut; Mv, microvilli; Nc, nerve cord; Pmg, posterior midgut; Sg, salivary glands. Bars, 100 nm.