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Derived cleaved amplified polymorphic sequence (dCAPS) marker analysis to detect the single-base deletion in the e1lb allele. (A) Primers designed and the generation of an HpyCH4IV restriction site. One of three cytosines marked by red was deleted in ZE. (B) Gel electrophoresis of PCR products without (–) or with (+) HpyCH4IV digestion. H-e3, Harosoy NIL for e3; ZE, Zeika.
