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. 2019 Jan 14;9:101. doi: 10.1038/s41598-018-36473-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative photomicrographs of TUNEL positive cells in the ganglion cell layer (GCL) in normal control, PBS and Triplet-treated rats at 5 days after ONC. (Scale Bar: 20 μm) (B) Graph showing the ratio of TUNEL-positive to the total number of retinal neuronal cells in the GCL after ONC. Compared with the untreated PBS group, TUNEL positive ratio was decreased significantly in the Triplet eye drop treatment group (**P < 0.001). Values represent means ± SEM of n = 3 animals per experimental group.