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. 2019 Jan 14;9:101. doi: 10.1038/s41598-018-36473-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Triplet instillation induces axonal regeneration after ONC. (A) Sagittal sections through the optic nerve showing GAP-43-labeled axons distal to the injury site (dotted line) in PBS and triplet-treated rats at 2 weeks after ONC. Arrowheads indicate regenerating axons. (Scale bar:100 µm) (B) Higher magnification of the images shown in figure A. (Scale bar:50 µm) (C) Graph showing quantification of regenerating axons at different distances distal to the lesion site (*P < 0.05). Values represent means ± SEM of n = 3 animals per experimental group.