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. 2019 Jan 14;9:93. doi: 10.1038/s41598-018-36500-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summary statistics of RNA sequencing data. (a) Unsupervised hierarchical clustering was performed for differentially expressed genes (absolute value of fold change >2 and FDR < 0.05) in the IL-1β treated cartilage explants. (b) Distribution of gene expression fold changes according to log₂ transformation. (c) Validation of RNAseq results with qRT-PCR. Seven selected genes were tested and correlated with the Pearson’s correlation analysis. (d) Enrichment analysis of the protein family classification and Gene Ontology annotations in terms of (e) molecular functions and (f) biological processes.