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. 2019 Jan 14;9:95. doi: 10.1038/s41598-018-36495-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE analysis of different recombinant proteins for longifolene biosynthesis expressed in E. coli. Recombinant protein expression was induced using 0.1 mM IPTG for a cultivation time of 4 h at 37 °C. Lane M, protein molecular weight marker; lane 1, control strain harboring pACYCDuet-1; lane 2, crude cells extracts from strain BL21/pA-lgfS; lane 3, crude cells extracts from strain BL21/pA-ispAlgfS; lane 4, crude cells extracts from strain BL21/pA-isoAlgfS; lane 5, crude cells extracts from strain BL21/pA-erg20lgfS; lane 6, crude cells extracts from recombinant strain harboring pTrcLower; lane 7, crude cells extracts from recombinant strain harboring both BL21/pA-mvaESispAlgfS and pTrcLower. The bands corresponding to the individual proteins were indicated by an arrow.