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. 2019 Jan 14;9:95. doi: 10.1038/s41598-018-36495-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of longifolene by GC-MS. (A) total ion current chromatogram of longifolene standard (the peak of longifolene was marked with an arrow corresponding to the retention time of 8.87 min). (B) total ion current chromatogram of the gas in the sealed shake flask from E. coli BL21(DE3) harboring pA-lgfS after being induced for 24 h. (C) mass spectrum of longifolene standard. (D) mass spectrum of the fermentation product. The structure of longifolene was matched by searching the NIST 08 library. The molecular ion peak of longifolene was marked with a triangle.