Table 1.
Strains and plasmids used in this study.
| Strains or plasmids | Genotype/Description | Sources |
|---|---|---|
| Strains | ||
| E. coli DH5α | F − recA endA1 Φ80dlacZΔM15 hsdR17(r K − m K + ) λ − | Invitrogen |
| E. coli BL21(DE3) | F− ompT hsdSB (rB− mB−) gal dcm rne131 (DE3) | Invitrogen |
| S. cerevisiae 288c | MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6 | American type culture collection (ATCC) |
| B. trispora DSM-2388 | Mating type - | German Collection of Microorganisms and Cell Cultures (DSMZ) |
| Plasmids | ||
| pACYCDuet-1 | Cm r oriP15A lacI q T7p | Novagen |
| pET30a(+) | Kan r oripBR322 lacI q T7p | Novagen |
| pEASY-Blunt | Kan r Amp r oripUC | Transgen |
| pTrcHis2B | Amp r ori pBR322 lacI q Trcp | Invitrogen |
| pUC-lgfS | pUC57 harboring codon optimized P. abies lgfS | This study |
| pET-lgfS | pET30a harboring P. abies lgfS | This study |
| pA-lgfS | pACYCDuet-1 harboring P. abies lgfS | This study |
| pEasy-ispA | pEASY-Blunt harboring E. coli ispA | This study |
| pEasy-isoA | pEASY-Blunt harboring B. trispora isoA | This study |
| pEasy-erg20 | pEASY-Blunt harboring S. cerevisiae erg20 | This study |
| pA-ispAlgfS | pACYCDuet-1 harboring E. coli ispA and P. abies lgfS | This study |
| pA-isoAlgfS | pACYCDuet-1 harboring B. trispora isoA and P. abies lgfS | This study |
| pA-erg20AlgfS | pACYCDuet-1 harboring S. cerevisiae erg20 and P. abies lgfS | This study |
| pTrcLower | pTrcHis2B harboring S. cerevisiae erg19, erg8, erg12 and idi1 | 30 |
| pA-mvaES | pACYCDuet-1 harboring E. faecalis mvaE and mvaS | 30 |
| pA-mvaESispA | pACYCDuet-1 harboring E. faecalis mvaE, mvaS and E. coli ispA | This study |
| pA-mvaESispAlgfS | pACYCDuet-1 harboring E. faecalis mvaE, mvaS, E. coli ispA and P. abies lgfS | This study |