Table 2.

Primers used in this study for plasmids construction.

Oligonucleotide primers	Sequences
lgfS_F_NdeI	GGGAATTCCATATGGCTCAGATCAGTAAATGCTC
lgfS_R_BglII	GGAAGATCTTAGGTCAGCGGGTCGATCAG
ispA_F_NcoI	CCATGGACTTTCCGCAGCAACTC
ispA_R_BamHI	GGATCCTTATTTATTACGCTGGATGATGTAGTCC
isoA_F_NcoI	CCATGGTTGCTGTCAAGTTACCAAAG
isoA _R_SalI	GTCGAC TTATTTAGTACGCTTGTAGATCTTG
erg20_F_NcoI	CCATGGCTTCAGAAAAAGAAATTAGGAG
erg20_R_BamHI	GGATCCCTATTTGCTTCTCTTGTAAACTTTGTTC
ispA_F_BglII	GGAAGATCTCATGGACTTTCCGCAGCAACTC
ispA_R_PvuI	TCGCGATCGTTATTTATTACGCTGGATGATGTAGTCC
T7lgfS_F_PvuI	TCGCGATCGTAATACGACTCACTATAGGGGAATTGTG
T7lgfS_R_XhoI	CCGCTCGAGTTAGGTCAGCGGGTCGATCAGGATTTTG

The restriction sites in the primers were underlined.