Fig. 2.

Retained B cells after anti-CD20 IgG₁ depletion are enriched for IgD^low/− expression. a Representative flow cytometry gating strategies in B10.PL mice are shown for splenic B220⁺ B cells differentiating T1, T2, T3, T2-MZP, FO, and MZ B cells utilizing differential expression of IgM, CD21, CD23, and CD93 14 days after administration of isotype (top row) or anti-CD20 IgG₁ (bottom row). b IgD expression was assessed on the FO B cell subset from a. c–i WT C57BL/6 mice were used to assess expression levels of IgD (c), IgM (d), B220 (e), CD20 (f), CD23 (g), CD21 (h), and CD93 (i) on MZ, BD_L, and FO B cells by flow cytometry. The mean fluorescence intensity ± SEM is shown from nine mice