Fig. 5.
BDL regulatory activity is GITRL-dependent. a Splenic T1, T2, T2-MZP, MZ, BDL, and FO B cells from B10.PL mice expressing GITRL was determined by flow cytometry. The data shown are the MFI ± SEM of three mice from one of four independent experiments. *p < 0.05, BDL vs. FO B cells. b–d C57BL/6μMT mice were reconstituted with 5 × 106 FACS-purified WT BDL (b, d), WT FO (b, d), Tnfsf18−/− (GITRL−/−) BDL (d) cells from C57BL/6 mice with (b) or without (d) GITRL antibody blocking and the absolute number of splenic Treg was determined by flow cytometry 10 days later. c Treg were quantitated in the spleen of WT and Tnfsf18−/− mice by flow cytometry. b Individual data points (mice) are shown superimposed upon the mean ± SEM from two independent experiments. **p < 0.01, μMT + WT BDL vs. μMT + GITRL blocked WT BDL; ***p < 0.001, μMT vs. μMT + WT BDL; ****p < 0.0001, WT vs. μMT. c Individual data points (mice) are shown superimposed upon the mean ± SEM of five mice. **p < 0.01. d **p < 0.01, μMT + WT BDL vs. μMT + Tnfsf18−/− BDL; ***p < 0.001, μMT vs. μMT + WT BDL; ****p < 0.0001, WT vs. μMT. Statistical significance was determined using the unpaired t test