Fig. 6.
BDL are mature and emerge from the T2 stage. a C57BL/6J mice were sublethally irradiated (500 rad) and the emergence of T2, FO, and BDL was tracked in the spleen on the days indicated by flow cytometry and the absolute number of cells was calculated. b C57BL/6J mice were lethally irradiated (950 rad) and transplanted with syngeneic BM and the emergence of T2, FO, and BDL was tracked in the spleen on the days indicated by flow cytometry and the absolute number of cells was calculated. a, b Individual data points (mice) are shown superimposed upon the mean ± SEM. c, d C57BL/6J CD45.1 mice were sublethally irradiated (500 rad) and 15 days later T2 cells were FACS purified, labeled with CFSE, and 1.25 × 106 cells/recipient were adoptively transferred to C57BL/6J CD45.2 mice and 24 and 48 h later the percentage (c) and absolute number (d) of T2, FO, and BDL was determined in the spleen by flow cytometry. T2, FO, and BDL B cell subsets were gated as for Fig. 2a, b. c Data shown are one representative experiment of three. d Individual data points (mice) are shown superimposed upon the mean ± SEM from one representative experiment of three. Statistical significance was determined using the unpaired t test