Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 31;13(2):237–249. doi: 10.1038/s41396-018-0261-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — Ectopic expression of the Rbp and chaperone is sufficient to retarget R-type syringacins. The results of overlays of six different strains and five different PEG-selected supernatants are shown. Overlay strains are shown on the y axis, whereas supernatants are shown on the x axis. Three strains are sensitive to killing by R-type syringacins produced by PsyB728a and not Pja (Psy301D, Pan, USA007), while three strains are sensitive to R-type syringacins produced by Pja and not PsyB728a (PtoDC3000, USA011, Por1_6). Killing activity is abolished in a mutant of PsyB728a where both the receptor-binding protein (Rbp) and chaperone from the R-type syringacin has been deleted, and this mutant can be complemented by the expression of both these genes ectopically from a plasmid. Ectopic expression of the Rbp and chaperone from Pja in this PsyB728a ΔRbp/chaperone background retargets this strain so that the killing spectrum matches that of Pja