Fig. 3.

Attraction to Z5–14:OH is diminished in Olfr288 knockout mice. a Series of control experiments in the two-choice odor-preference test. Intact: urine from intact male C57BL/6 mice; Cast.: urine from castrated male C57BL/6 mice. TMT was diluted in PG. Oil: mineral oil; EG: eugenol. b, c Attraction to various amounts of Z5–14:OH of adult female C57BL/6 mice (N = 22–24) (b) and adult female Olfr288-KO mice (N = 18–20) (c). Z5: Z5–14:OH. d, e Preference for castrated male urine, with or without various amounts of Z5–14:OH, of adult female C57BL/6 mice (N = 20–24) (d) and adult female Olfr288-KO mice (N = 16–23) (e). f Preference for intact male urine vs. castrated male urine in adult female Olfr288-KO mice (N = 19). Bars indicate the mean time that a mouse spent investigating each test sample within a 3-min period, ±S.E.M. (N = 16–29). Asterisks indicate significant difference between two samples (paired Student’s t-test, *P < 0.05, **P < 0.01)