Fig. 5.

147 acts transiently in vivo. a qPCR for Atf6 and ATF6 target genes Grp78, Grp94, and Cat in WT (n = 5) mice either 8 hours, 1 day, or 7 days after a single bolus venous injection of 147 (2 mg/kg). b 147 plasma concentration as a function of time in mice receiving 2 mg/kg via venous injection. Blood was collected at baseline and 5min, 15min, 30min, 1h, 2h, and 4h post injection (n = 4 mice per timepoint). Data are represented as mean ± s.e.m. Two-group comparisons were performed using Student’s two-tailed t test, and all multiple group comparisons were performed using a one-way ANOVA with a Newman–Keuls post hoc analysis. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001; ns not significant