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. 2019 Jan 14;9:94. doi: 10.1038/s41598-018-36993-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of SHANK3 gene expression in iPSC-derived neurons from controls and ASD patients. (a) Quantification of SHANK3 mRNA in iPSC-derived neurons (40 days post NSC) using RT-ddPCR. Data are mean ± SEM. Statistical analysis was performed using a two-way Anova. *p < 0.05 ***p < 0.001 (b) Sequencing diagrams showing the presence of the four mutations in the genomic and cDNA extracted from iPSC-derived neurons.