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. 2019 Jan 14;9:94. doi: 10.1038/s41598-018-36993-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation analysis between SHANK3 expression and alterations in dendritic spine parameters in iPSC-derived neurons from control individuals and patients with ASD. Upper graph, Correlation between SHANK3 mRNA and spine densities. Middle graph, Correlation between SHANK3 mRNA and total spine volume. Lower graph, Correlation between SHANK3 mRNA and head spine volume. Statistical analysis was performed using GraphPad Prism Version 6 software (GraphPad, sand Diego, California, USA). Data are mean ± SEM. Coefficients were calculated using Spearman correlation method and are indicated in the graphs with statistical significance.