Fig. 4.

TM inhibition of the protein aggregation induced by cisplatin. a, b CD spectra of WLN4 (a) or Cu-WLN4 (b). TM-WLN4 complexes were prepared by reaction of the protein with 3 eq. TM for 3 h at 37 °C. The protein platination was performed by incubation with 3-molar equivalents of cisplatin for 8 h. c–f Gel electrophoresis analysis of the protein aggregation inhibited by TM. c Cu-MNK3; d Cu-WLN4; e apo-MNK3; f apo-WLN4. The copper protein was prepared by incubation with 1.5-fold Cu for 15 min at room temperature. Proteins (200 µM) were reacted with TM (600 µM) for 3 h, then were incubated with cisplatin (600 µM) for 2 h. Reactions were performed in 50 mM MES, 150 mM NaCl, pH 6.0 at 37 °C. Symbols +/- indicate presence/absence of the species