Figure 7.
CTCF is required for CIN distribution across cortical layers. A, D, G, I, ISH of P21 cortices with the indicated probes. B, E, H, J, Quantification of cortical cell densities. Error bars indicate mean ± SEM. C, F, The cortex was divided into 10 horizontal bins, where Bin 1 represents the most superficial layer. Two-way repeated-measures ANOVA was significant for both Gad1 and Reelin. Student's t test was performed for each bin and significantly different bins (p < 0.05) are marked by asterisks (not corrected for multiple testing). K, Immunofluorescence staining for SP8 (red) in P21 cortex counterstained with DAPI. Merged blue and red channels are shown. Scale bar, 100 μm. Bottom inset shows higher magnification of SP8+ cells. L, Quantification of SP8+ cells. Error bars indicate mean ± SEM, n = 3 pairs. p = 0.432, Student's t test.