Skip to main content
. 2019 Jan 15;39(1):BSR20181859. doi: 10.1042/BSR20181859

Figure 3. GAS5 was a direct target of miR-221/222.

Figure 3

(A and B) Quantitative RT-PCR analysis of miR-221/222 levels in MCF-7, MDA-MB-231 and SKBR3 cells transfected with equal doses of premiR-221/222, anti-miR-221/222 or scrambled negative control RNAs (pre-miR-control or anti-miR-control). (C) Firefly luciferase reporters containing either wild-type (WT) or mutant (MUT) miR-221/222 binding sites in the GAS5 3′-UTR were co-transfected into HEK293T cells along with equal doses of pre-miR-221/222 or pre-miR-control. The cells were assayed using a luciferase assay kit 24 h post-transfection. Firefly luciferase values were normalized to β-gal activity, and the results were calculated as the ratio of firefly luciferase activity in the miR-221/222-transfected cells normalized to the premiR-control-transfected cells; **P<0.01; ***P<0.001.