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. 2018 Nov 27;6(1):129–143. doi: 10.1002/acn3.687

Figure 2.

Figure 2

IVIg‐induced plasmablasts are enriched for IgG2. Intracellular staining of immunoglobulin classes and subclasses was performed on cryo‐preserved PBMC from GBS patients (n = 20) and healthy controls (n = 20). Plasmablasts (CD19+/low CD27+ CD38+) were gated and expression of all classes and subclasses was observed (A; top panels pretreatment and lower panels posttreatment). No difference was found in IgM (B), IgA (C), and IgG (D) positive plasmablasts before and 1 week after IVIg treatment. Quantification of IgG1 (E), IgG2 (F), and IgG3 (G) subclasses demonstrated a significant shift in IgG subclass usage 1 week after IVIg treatment. Samples with few plasmablasts were excluded. IGG transcripts analyzed by 454 sequencing also indicated a significant shift in unique IGG subclass sequences in GBS patients (n = 3) 1 week posttreatment (H; two‐way ANOVA followed by Bonferroni correction). The fraction of sequences found in IGG2 only, and not shared with other (sub)classes was also increased after treatment (I; data represent means of three patients). *P < 0.05; **P < 0.01; ***P < 0.001.